Cyclic AMP Signaling is Required for Function of the N-terminal and CR1 Domains of Adenovirus E1A in Saccharomyces cerevisiae

Mary E. Miller, Daniel A. Engel, & M. Mitchell Smith [1]

Department of Microbiology and University of Virginia Cancer Center Box 441 Jordan Building, School of Medicine, University of Virginia Charlottesville, VA 22908

Oncogene 11, 1623-1630 (1995)

Table of Contents

• Abstract
  
• Introduction
  
• Results
  
  • Mapping functional domains of E1A
    
  • Protein expression levels
    
  • Nuclear localization
    
  • Residues 1-82 are sufficient for function
    
  • Yeast mutants resistant to E1A
    
  • Identification of two NET genes: adenylate cyclase and the Ras GTP-GDP exchange factor Cdc25p
    
• Discussion
  
• Materials and methods
  
  • Strains
    
  • Plasmids
    
  • Isolation of Mutants
    
  • Phenotypic Analysis
    
• Acknowledgments
  

Copyright 1995 Stockton Press All rights reserved 0950-9232/95