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University of Virginia  

The structure & function of macromolecular complexes
We are interested in protein-protein and protein-DNA complexes. The main tools that we employ are electron microscopy and computational image analysis. Our long-standing interests have involved F-actin and RecA/Rad51 recombination filaments. However, our development of new methods for the analysis and reconstruction of helical polymers has led us to apply these tools to many other filamentous assemblies, such as bacterial pili, Type Three Secretion System components, and filamentous bacteriophage. We now have the ability to reach atomic and near-atomic resolutions almost routinely from many helical polymers.