Edward Perez-Reyes

Professor of Pharmacology

University of Virginia

 

 

Calcium entry via voltage-gated calcium channels is a key event in neuronal firing, muscle contraction, gene expression, and secretion of neurotransmitters and hormones. Importantly, voltage-gated calcium channels are the site of action of a number of clinically relevant drugs, termed "calcium channel blockers."

The major contribution of the Perez-Reyes laboratory has been on the molecular physiology and pharmacology of voltage-gated calcium channels, and in particular, the cloning of low voltage-activated T-type calcium channels.  Their cloning opened many doors on their biophysics, localization, role in neuronal excitability, and pharmacology. This work combined a wide array of techniques such as molecular cloning, electrophysiological recordings of whole cell and single channel currents, and confocal microscopy.  For review see: “Molecular physiology of low-voltage-activated T-type calcium channels” Physiol. Rev. 83:117-161, 2003, PMID: 12506128.  Our T-channel studies continue to focus on how mutations in Cav3.2 alter its function leading to diseases such as epilepsy. Our most current study on the mechanisms by which Cav3.2 mutations found in childhood absence epilepsy patients lead to hyperexcitability (J. Physiol. PMID: 24277868).

 

Current work in the lab is focused on developing gene therapies for pharmacoresistant epilepsies.  In these projects we are leveraging our experience in molecular cloning to develop novel adeno-associated viral (AAV) targeting vectors capable of delivering either short-hairpinned RNAs (shRNAs) or genes of interest. Figure below shows drug-inducible delivery of two genes from a single adeno-associated virus in the brain (GFP and mCherry).  In a project funded by a CURE Challenge Award, we validated the ability of a modified K+ leak channel to reduce status epilepticus (Epilepsia: PMID: 24299204).   In a second project recently funded by NINDS, we are testing the ability of an shRNA directed against the sodium channel subunits to reduce seizures. Both projects are made possible by collaborations with local epilepsy experts, including Jaideep Kapur, John Williamson, Edward Bertram, Mark Beenhakker, and my closest collaborator, Manoj Patel.

 

We find these new translational neuroscience projects very exciting and hope they will have a major impact on patient care. 

 

Current lab members:

Iuliia Vitko, Instructor

Deborah L. Perez-Reyes, Visiting Lecturer

Kyle Sullivan, UVa Undergraduate Student

Kathyrn Brodie, UVa Undergraduate Student

 

Former lab members who left in the last few years:

Alex Shcheglovitov, former Post-doc, now an Assistant Professor at the University of Utah.

Veit Simon-Eckle, former post-doc now at the University of Tubingen

Joel Baumgart, former Ph.D. Student, now at Weill Cornell Medical College

Amol Bhandare, former Research Assistant, now seeking Ph.D. degree

Conor Jennings, UVa Undergraduate Student, now seeking M.S. degree

Everardo Hernandez-Plata, Visiting Ph.D. student from UNAM, now post-doc at the Imperial College of London.

Deblina Dey, former Ph.D. student, now post-doc at U.C.-Irvine.

For a complete list of scientific contributions see my profile on Google Scholar: perez-reyes e

Contact eperez@virginia.edu

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Last updated November 15, 2013
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