David Auble
The images presented here were derived from 1tbp and 1ytb. The 1ytb co-crystal structure shows TATA-binding protein (TBP) bound to a short, TATA-containing hairpin DNA. To more clearly demonstrate the remarkable distortion of DNA induced by TBP binding, B-form duplex DNA was appended to the ends of the original structure for some of the images in this display. Note the sharp change in DNA trajectory (nearly 90 degree bending) induced by intercalation of phenylalanine residues between base pair steps at either end of the TATA Box. Note also the unwinding of TATA DNA that provides a complementary hydrophobic surface for interaction between the minor groove and the underside of the TBP 'saddle'. The original co-crystal structure was used in images that depict interactions between TBP and DNA.
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The TBP-DNA complex forms a scaffold for assembly of other general transcription factors at the promoter. If you're interested in seeing how other proteins interact with the TBP-DNA complex, take a look here.
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