David Auble, 3-2629, dta4n@virginia.edu
Reading and handouts
Lecture handouts and related material are available from the Collab site.
Protein purification
Click the links to view animations showing how gel filtration, ion exchange, hydrophobic interaction, and affinity chromatography work.
Optional: Lots of information about chromatography, including how to set up a protein purification scheme and descriptions of various chromatography media and protocols for their use, can be found at the GE Healthcare protein purification site .
View macromolecular structure
Visit this site where you can view and manipulate structures of protein-DNA and protein-protein complexes discussed in class. There is also accompanying text that reinforces the main points of the lectures.
Browse the compendium of protein-DNA structures and see other neat stuff here.
Structures of myoglobin and hemoglobin, plus a tutorial highlighting structural features of these proteins can be viewed here (highly recommended). Optional: A recent well-written minireview on hemoglobin and cooperativity (Ackers and Holt, J. Biol. Chem. 281:11441-11443 (2006)) is also available from the password-protected site.
Visit the Database of Macromolecular Movements to see additional movies of proteins undergoing conformational changes. (Check out hemoglobin, for instance.)