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•UVA Department of Biochemistry & Molecular Genetics •UVA Biomolecular Core Facility •David's lectures in BIMS/Biochemistry 503
•Auble Lab resources (password protected)
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Research Overview We are interested in the fundamental question of how transcription complexes are assembled on promoter DNA and how their activities are regulated. This regulation underpins cellular responses to changes in the environment, developmental cues, and disease states such as cancer. Our focus is on factors that regulate the activity of the TATA-binding protein (TBP), a component at the heart of the eukaryotic multi-subunit transcription preinitiation complex (PIC). Although TBP can bind to promoters very stably in vitro, its binding to chromatin is highly dynamic in vivo. This high mobility is a consequence of the catalytic activity of Mot1, an essential ATPase that is a member of the Snf2/Swi2 enzyme family. Our current goals are to understand why TBP's highly dynamic activity is essential for cell viability, what roles TBP displacement from DNA play in gene regulation, and how Mot1 catalyzes this interesting reaction. Lab meeting schedule- spring/summer '11 4/20 Melissa
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•Saccharomyces Genome Database Pattern matching/cis element detection: Venn diagrams: GO term analysis: |