CURRICULUM VITAE

David S. Cafiso, Professor

Education:
Univ. of California, Berkeley, Calif. A.B. 1974 Biophysics
Univ. of California, Berkeley, Calif. Ph.D. 1979 Biophysics
Univ. of California, Berkeley, Calif. 1979 - 1980 Postdoctoral Study
Stanford University, Stanford, Calif. 1980 - 1981 Postdoctoral Study

Employment/Research Appointments:
1975-1976 Research Assistant, Department of Molecular Biology, Univ. of California, Berkeley. Research: isolation and characterization of heat sensitive E. coli DNA polymerase mutants (mentor Donald Glaser)
1976-1979 Research Assistant, Department of Chemistry, Univ. of California, Berkeley. Research: the synthesis and development of EPR probes of membrane potential and the investigation of photoreceptor electrostatics (mentor: Wayne L. Hubbell).
1979-1980 Postdoctoral Fellow of the Jane Coffin Childs Memorial Fund for Medical Research, Department of Chemistry, Univ. of California, Berkeley. Research: the investigation of the ion selective properties of photoreceptor and model membrane systems (mentor: Wayne L. Hubbell).
1980-1981 Postdoctoral Fellow of the Jane Coffin Childs Memorial Fund for Medical Research, Department of Chemistry, Stanford Univ. Research: investigation of the structure and dynamics of cell and model membrane systems (mentor: Harden M. McConnell).
1981-1987 Assistant Professor of Chemistry at the Univ. of Virginia.
1987-1992 Associate Professor of Chemistry at the Univ. of Virginia.
1992-2008 Professor of Chemistry at the Univ. of Virginia.
2007-Present  Professor of Molecular Physiology and Biological Physics, University of Virginia School of Medicine (joint appointment).
2008-2011  Chair, Department of Chemistry, University of Virginia
2008-present.  Alfred Burger Professor of Biological Chemistry, University of Virginia.  Research activities include investigating the molecular basis of membrane transport and the molecular interactions regulating cell signaling and membrane trafficking. Teaching responsibilities have included physical chemistry, biochemistry, and spectroscopy. Total of 24 Ph. D. and 11 M.S. theses directed.

Societies/Service/Honors:
Elected Fellow, Biophysical Society, 2010.
Jane Coffin Childs Foundation Fellow, 1979-1981.
Camille and Henry Dreyfus Award for Distinguished New Faculty in Chemistry, 1981.
Member, NSF Biophysics Panel, 1990-1993. Member, NIH IRG Study Section.
Adhoc member, NIH BBCB study section, 1996-present.
NIH Biomembranes Study Section, 2005.
Editorial Board, Biophysical Journal.
Editorial Board, Journal of Liposome Research.
Editorial Board, Cell Biochemistry and Biophysics.
Biophysical Society: 1978-present;
American Society for the Advancement of Science: 1980 - present.
American Chemical Society: 1981 - present.
Faculty of 1000 contributor, 2001- present.

Selected Research Accomplishments:

Development of molecular probes for measuring membrane electrostatics.
We developed molecular probes based on spin-labels to measure transmembrane, dipolar and interfacial potential in biological membranes. Ann. Revs. Biophys. Bioeng. 10:217 (1981).

Determination of the molecular source for the early receptor potential.
An interfacial charge transfer in event in rhodopsin was characterized and quantitated. It results from the deprotonation of an acidic residue within the interface and is the molecular event is the source of the early receptor potential (see: Photochemistry and Photobiology 32:461 (1980) and Biophys. J. 30, 243 (1980)).

First use of 2 dimensional NMR to map 1H relaxation pathways and determine small molecule location in lipid bilayers.
We reported the first applications of 2D NMR to investigate spin diffusion and NOEs in model membranes. These measurements provided a picture for lipid conformations in membranes, and gave a remarkable view of the extreme conformations that are present. It also allowed measurements to localize small molecules and peptides within bilayers (see: J. Am. Chem. Soc. 107:1530-1537 (1985); Biophysical Journal, 49:779-783 (1986)).

First use of mass spectrometry to determine membrane protein topology.
We applied triple quadupole and tandem FT mass spectrometry combined with proteolysis and selective labeling to determine the transmembrane topology of the nicotinic acetylcholine receptor (see: Biochemistry 28, 9184-9191 (1989)).

First use of site-directed spin labeling to map protein-protein interactions.
Site-directed spin labeling and EPR spectroscopy were used to determine the molecular interactions between calmodulin and two target proteins, MARCKS and neuromodulin (see: Biochemistry 35, 13272-13276 (1996)).

Determination of the structures and interactions controlling the association of basic protein domains with membrane interfaces.
Characterized the structures and forces responsible for the association of positively charged protein domains with membrane interfaces. A balance of long-range attractive and short-range repulsive forces determines the equilibrium position of these domains. This position is important in proteins such as MARCKS, which we hypothesize functions to sequester PI(4,5)P2 within the plane of the bilayer. (see: Biochemistry 38, 12527-12536 (1999); J Biol. Chem. 277, 14068-76 (2002)).

Discovery and characterization of a substrate-induced conformational gating of a membrane transporter.
We elucidated the first molecular step in the transport process for the movement of vitamin B12 through the outer membrane transporter BtuB. These measurements used site-directed spin labeling in both native and reconstituted membranes (see: Nature Struct. Biol. 7, 205-209 (2000); Biochemistry 42, 1391-1400 (2003)).

First determination of the docking of the C2 domains of synaptotagmin to membrane interfaces.
We determined the membrane position of the two C2 domains from synaptotagmin on membrane surfaces. These domains play a critical role in Ca2+-dependent neuronal exocytosis. These membrane positions provide insight into the forces that regulate Ca2+-binding and are a starting point for determining their role in regulated exocytosis (see: Biochemistry, 42, 96-105 (2003)).

Demonstration that osmolytes can lead to conformational trapping in protein crystal structures.
We recently demonstrated that solutes that are used to crystallize proteins can modulate conformational changes that involve changes in hydration (see Biochemistry 42, 13106-13112 (2003)).

Determining the molecular mechanism of transmembrane-signaling in a membrane transporter.  Using site-directed spin labeling in a novel way with a two-mutant cycle analysis, we demonstrated that the weakening of a salt-bridge within the outer-membrane vitamin B12 transporter, BtuB, was the molecular trigger for a substrate-dependent transmembrane signaling event (see: J. Mol. Biol. 370, 807-811 (2007)).

First membrane-bound structure for the Ca2+-sensor in neuronal exocytosis.  Using EPR-derived distance restrains with a modified simulated annealing program, we determined the membrane bound configuration for synaptotagmin 1, the calcium-sensor in neuronal exocytosis.  The data indicate that this protein functions to bridge bilayers in the presence of calcium (see: J Mol Biol. 2009 390, 913-23 (2009))

Publications:

Thesis:

Electrical and Ion Selective Properties of Photoreceptor Membranes. University of California, Berkeley, May 1979.

Papers:

Estimation of Transmembrane Potentials from Phase Equilibria of Hydrophobic Paramagnetic Ions. D. S. Cafiso and W. L. Hubbell. Biochemistry 17:187 (1978).

Estimation of Transmembrane pH Gradients from Phase Equilibria of Spin Labeled Amines. D. S. Cafiso and W. L. Hubbell. Biochemistry 17:3871 (1978).

Light-Induced Interfacial Potentials in Photoreceptor Membranes. D. S. Cafiso and W. L. Hubbell. Biophysical Journal 30:243 (1980).

Interfacial Charge Separation in Photoreceptor Membranes. D. S. Cafiso and W. L. Hubbell. Photochemistry and Photobiology 32:461 (1980).

An Additional Component Required for Activity and Reconstitution of Light-Activated Vertebrate Photoreceptor GTPase. T. Shinazawa, S. Uchida, E. Marin, D. Cafiso, W. Hubbell and M. Bitensky. Proc. Natl. Acad. Sci. 77:1408 (1980).

ESR Determination of Membrane Potentials. D. S. Cafiso and W. L. Hubbell. Ann. Revs. Biophys. Bioeng. 10:217 (1981).

Investigations of the Electrostatic Structure of Membranes: New Approaches With Spin-Labeling. D. S. Cafiso and W. L. Hubbell. in Molecular Basis of Drug Action, Singer and Ondarza, Eds. (1981) p253.

Preparation of Unilamellar Lipid Vesicles at 37°C by Vaporization Methods. D. S. Cafiso, H. R. Petty and H. M. McConnell. Biochim. Biophys. Acta 649:129 (1981).

Spin-Label Probes of Light-Induced Electrical Potentials in Rhodopsin and Bacteriorhodopsin. D. S. Cafiso, A. Quintanilha, W. L. Hubbell, in Methods in Enzymology series on "Visual Pigments and Purple Membranes" Vol. 88, 682 (1982).

Transmembrane Electrical Currents of Spin-Labeled Hydrophobic Ions. D. S. Cafiso and W. L. Hubbell. Biophysical Journal 39:263-272 (1982).

Electrogenic H+/OH- Movement Across Phospholipid Vesicles Measured by Spin-Labeled Hydrophobic Ions. D. S. Cafiso and W. L. Hubbell. Biophysical Journal 44:49 (1983).

Paramagnetic Hydrophobic Ions as Probes for Electrically Active Conformational Transitions in Ion Channels. D. S. Cafiso. Biophysical Journal 45:6-7 (1984).

Potential-Dependent Phase Partitioning of Fluorescent Hydrophobic Ions. D. E. Raines and D. S. Cafiso. Journal of Membrane Biology 82:241-247 (1984).

Elucidation of Cross-Relaxation Pathways in Phospholipid Vesicles Utilizing Two-Dimensional 1H NMR Spectroscopy. J. E. Ellena, W. C. Hutton and D. S. Cafiso. J. Am. Chem. Soc. 107:1530-1537 (1985).

ESR Methods for the Determination of H+/OH- Movement Across Phospholipid Vesicles. D. S. Cafiso, in Protons and Water: Structure and Translocation. Methods in Enzymology, Biomembranes. 127:502-510 (1986).

Electrokinetic and Electrostatic Properties of Bilayers Containing Gangliosides GM1, GD1a or GT1: Comparison with Non-Linear Theory. R. V. McDaniel, K. Sharp, D. Brooks, A. C. McLaughlin, A. P. Winiski, D. S. Cafiso and S. McLaughlin. Biophysical Journal 49:741-752 (1986).

Reconstitution of an Electrically Active Conformational Transition in Rhodopsin Containing Membranes. D. S. Cline and D. S. Cafiso. Biochim. Biophys. Acta 854:151-155 (1986).

Phospholipid Packing and Conformation in Small Vesicles Revealed by Two-Dimensional 1H-NMR Cross-Relaxation Spectroscopy. Z. Xu and D. S. Cafiso. Biophysical Journal, 49:779-783 (1986).

An Electrical and Structural Characterization of H+/OH- Currents in Phospholipid Vesicles. W. R. Perkins and D. S. Cafiso. Biochemistry 25:2270-2276 (1986).

Molecular Dynamics in Dodecyl Sulfate Micelles Elucidated Using 13C and 1H Spin-Lattice Relaxation, 13C Spin-Spin Relaxation and 1H Nuclear Overhauser Effect Spectroscopy. J. F. Ellena, R. N. Dominey and D. S. Cafiso. J. Phys. Chem., 91:131-137 (1987).

A Test of Discreteness-of-Charge Effects in Phospholipid Vesicles: Measurements Using Paramagnetic Amphiphiles. S. C. Hartsel and D. S. Cafiso. Biochemistry 25:8214-8219 (1987).

Time-Dependent Binding of Paramagnetic and Fluorescent Hydrophobic Ions to the Acetylcholine Receptor from Torpedo. S. C. Hartsel, C. R. Moore, D. E. Raines and D. S. Cafiso. Biochemistry 26:3253-3260 (1987).

A Procedure Using Voltage-Sensitive Spin-Labels to Monitor Dipole Potential Changes in Phospholipid Vesicles: The Estimation of Phloretin-Induced Conductance Changes in Vesicles. W. R. Perkins and D. S. Cafiso. J. Membr. Biol. 96:165-173 (1987).

The Localization of Hydrophobic Ions in Phospholipid Bilayers Using 1H Nuclear Overhauser Effect Spectroscopy. J. F. Ellena, R. N. Dominey, S. J. Archer, Z-C. Zu and D. S. Cafiso. Biochemistry 26:4584-4592 (1987).

Characterization of H+/OH- Currents in Phospholipid Vesicles. Walter R. Perkins and David S. Cafiso. J.Bioenerg. Biomembr. 19:443-455 (1987).

A Selective Cholesterol-Dependent Induction of H+/OH- Currents in Phospholipid Vesicles by Amphotericin B. S. C. Hartsel, W. R. Perkins, G. J. McGarvey and D. S. Cafiso. Biochemistry 27:2656-2660 (1988).

Localizing the Nitroxide Group of Fatty Acid and Voltage-Sensitive Spin-Labels in Phospholipid Bilayers. J. F. Ellena, S. J. Archer, R. N. Dominey, B. D. Hill and D. S. Cafiso. Biochim. Biophys. Acta 940:63-70 (1988).

The Enhancement of Proton/Hydroxyl Flow Across Lipid Vesicles by Inhalation Anesthetics. D.E. Raines and D.S. Cafiso. Anesthesiology 70:57-63 (1989).

Measuring Electrostatic Potentials Adjacent to Bilayer Membranes: Effects on Transport. D. Cafiso, A. McLaughlin, S. McLaughlin and A. Winiski, in Biomembranes and Biological Transport, Methods in Enzymology 171:342-364 (1989).

EPR Methods for Measuring pH Gradients, Transmembrane Potentials and Membrane Dynamics. D. S. Cafiso, in Biomembranes and Biological Transport, Methods in Enzymology 172:331-345 (1989).

Proteolytic Fragments of the Nicotinic Acetylcholine Receptor Identified by Mass Spectrometry: Implications for Receptor Topography. C. R. Moore, J. R. Yates, III, P. R. Griffin, J. Shabanowitz, P. A. Martino, D. F. Hunt and D. S. Cafiso. Biochemistry 28, 9184-9191 (1989).

Electrostatics of Phosphoinositide Bilayer Membranes: Theoretical and Experimental Results. M. Langer, D. Cafiso, S. Marcelja and S. McLaughlin. Biophysical J. 57, 335-349 (1990).

Lipid Bilayers: Membrane-Protein Electrostatic Interactions. D.S. Cafiso. Current Opinion in Structural Biology 1, 185-190 (1991).

A Voltage-Dependent Conductance for Alamethicin in Phospholipid Vesicles: A Test for the Mechanism of Gating. S. J. Archer and D. S. Cafiso. Biophysical Journal 60, 380-388 (1991).

Dynamics and Aggregation of the Peptide Ion Channel Alamethicin: Measurements using Spin-Labeled Peptides. S. J. Archer, J. F. Ellena and D. S. Cafiso Biophysical Journal 60, 389-398, (1991).

A Comparison of the Lipid Acyl Chain Dynamics in Small and Large Unilamellar Vesicles. L.S. Lepore, J. F. Ellena and D. S. Cafiso. Biophysical Journal 61, 767-775 (1992).

A Determination of the Molecular Dynamics of Alamethicin Using 13C NMR: Implications for the Mechanism of Gating of a Voltage-Dependent Channel. L.P. Kelsh, J. F. Ellena and D.S. Cafiso Biochemistry 31, 5136-5144 (1992).

Probes of Membrane Electrostatics: Synthesis and Voltage-Dependent Partitioning of Negative Hydrophobic Ion Spin-Labels in Lipid Vesicles. C. F. Franklin, D. S. Cafiso, R. Flewelling, and W. L. Hubbell Biophysical J. 64, 642-653 (1993).

Internal Electrostatic Potentials in Bilayers. Measuring and Controlling Dipole Potentials in Lipid Vesicles. J. C. Franklin and D. S. Cafiso. Biophysical J. 65, 289-299. (1993)

Estimating Lipid Lateral Diffusion in Phospholipid Vesicles from 13C Spin-Spin Relaxation. J. F. Ellena, Leslie S. Lepore, and David S. Cafiso J. Phys. Chem. 97, 2952-2957 (1993).

Alamethicin: a Peptide Model for Voltage-Gating and Protein-Membrane Interactions. D. S. Cafiso Annual Review of Biophysics and Biomolecular Structure 23, 141-165 (1994).

The Structure of Micelle Associated Alamethicin from 1H NMR. Evidence for Conformational Heterogeneity in a Voltage-Gated Peptide. J. C. Franklin, J. F. Ellena, S. Jayasinghe, L. P. Kelsh and D. S. Cafiso. Biochemistry 33, 4036-4045 (1994).

Collisions Between Helical Peptides in Membranes Monitored Using Electron Paramagnetic Resonance. Evidence that Alamethicin is Monomeric in the Absence of a Membrane Potential. M. Barranger and D. S. Cafiso. Biophysical Journal 67, 172-176 (1994).

Molecular Flexibility Demonstrated by Paramagnetic Enhancements of Nuclear Relaxation. Application to Alamethicin, a Voltage-Gated Peptide Channel. C. L. North, J. C. Franklin, R. G. Bryant, and D. S. Cafiso. Biophysical Journal 67, 1861-1866 (1994).

Experimental Determination of the Topography of Membrane Proteins: Lessons from the Nicotinic Acetylcholine Receptor, a Multisubunit Polytopic Protein, D. S. Cafiso, in Membrane Protein Structure: experimental approaches, S. White Editor, Oxford University Press (1994).

Anesthetics Reduce the Magnitude of the Membrane Dipole Potential. Measurements in Lipid Vesicles Using Voltage-Sensitive Spin-Probes. Zhihai Qin, Gabor Szabo, and David Cafiso Biochemistry 34, 5536-5543 (1995).

Distribution of General Anesthetics in Phospholipid Bilayers Determined Using 2H NMR and 1H-1H NOE Spectroscopy. James Baber, Jeffrey F. Ellena, David S. Cafiso. Biochemistry 34, 6533-6539 (1995).

Influences of Charges and Dipoles on Macromolecular Adsorption and Permeability. D. S. Cafiso. In Permeability and Stability of Lipid Bilayers. Simon and Disalvo eds. CRC press, Boca Raton (1995).

Antigen Based Heteropolymers Facilitate, Via Primate Erythrocyte Complement Receptor-Type-1, Rapid Erythrocyte Binding of an Autoantibody and its Clearance from the Circulation in Rhesus Monkeys. P. J. Ferguson, E. N. Martin, K. L. Greene, S. Kuhn, G. H. Addona, D. S. Cafiso, and R. P. Taylor. Journal of Immunology, 155, 339-347 (1995)

Membrane Orientation of the N-terminal Segment of Alamethicin Determined by Solid-State 15N NMR. C. L. North, M. Barranger-Mathys and D. Cafiso, Biophysical Journal 69, 2392-2397 (1995).

Membrane Structure of Voltage-Gated Channel Forming Peptides by Site-Directed Spin-Labeling. M. Barranger-Mathys and Cafiso, D. S. Biochemistry, 35, 498-505 (1996).

Membrane Structure of the PKC and Calmodulin Binding Domain of MARCKS Determined by Site-Directed Spin-Labeling. Q, Zhihai, and D. S. Cafiso Biochemistry, 35, 2917-2925 (1996).

Solution and membrane bound structure of a peptide derived from the protein kinase C substrate domain of neuromodulin. Wertz, S. L. Savino, Y. and D. S. Cafiso Biochemistry 35, 11104-11112 (1996).

Defining protein-protein interactions using site-directed spin-labeling: the binding of protein kinase C substates to calmodulin. Qin, Z, Wertz, S. L., Jacob, J., Savino, Y. and D. S. Cafiso Biochemistry 35, 13272-13276 (1996).

Contrasting Membrane Localization and Behavior of Halogenated Cyclobutanes that Follow or Violate the Meyer-Overton Hypothesis of General Anesthetic Potency. C. L. North and D. S. Cafiso Biophys. J. 72, 1754-1761 (1997).

MARCKS, Membranes, and Calmodulin: Kinetics of Interaction. Arbuzova, A., Wang, J. Murray, D., Jacob, J., Cafiso, D., McLaughlin, S. J. Biol. Chem. 272, 27167-27177 (1997).

Estimating the Electrostatic Potential at the Acetylcholine Receptor Agonist Site Using Power Saturation EPR. George H. Addona and David S. Cafiso. BBA Biomembranes 1329, 74-84 (1997).

Water Translational Motion at the Bilayer Interface: an NMR Relaxation Dispersion Measurement. Hodges, M., Cafiso, D., Polnaszek, C., Lester, C., and Bryant, R. Biophysical J. 73, 2575-2579 (1997).

Structure and Position of the N-terminal Binding Domain of pp60src at the Membrane Interface. Victor, K., and D. S. Cafiso. Biochemistry 37, 3402-3410 (1998).

Structural Features that Modulate the Transmembrane Migration of a Hydrophobic Peptide in Lipid Vesicles. Jayasinghe, S., Barranger-Mathys, M., Ellena, J. F., Franklin, C., and D. S. Cafiso. Biophysical J. 74, 3023-3030 (1998).

Influence of Lipid on the Structure and Phosphorylation of Protein Kinase C a Substrate Peptides. Vinton, B. Wertz, S., Jacob J., Steer, J., Grisham, C. Cafiso, D. and J. Sando. Biochemical Journal. 330, 1433-1442 (1998).

Dipole potentials and spontaneous curvature: membrane properties that could mediate anesthesia. David S. Cafiso. Toxicology Letters, 103, (1998).

The Interaction of Natural and Model Peptides with Membranes. D. S. Cafiso. In "Membrane Permeability: 100 Years Since Ernest Overton," D. W. Deamer Ed. Oxford University Press (1999).

The Role of Proline and Glycine in Determining the Backbone Flexibility of a Channel Forming Peptide. Jacob, J., Duclohier, H. and D. S. Cafiso. Biophysical Journal 76, 1367-1376 (1999).

Membrane Spontaneous Curvature Modulates the Binding Energy of a Channel Forming Voltage-Gated Peptide. Lewis, J. R. and D. S. Cafiso. Biochemistry 38, 5932-5938 (1999).

Distance Estimates from Paramagnetic Enhancements of Nuclear Relaxation in Linear and Flexible Model Peptides. Jabob, J., Baker, B., Bryant, R. G. and D. S. Cafiso. Biophysical Journal 76, 1086-1092 (1999).

Interactions Controlling the Membrane Binding of Basic Protein Domains. Phenylalanine and the Attachment of the MARCKS Protein to Interfaces. K. Victor and D. S. Cafiso. Biochemistry 38, 12527-12536 (1999).

Reconstitutive Refolding of Diacylglycerol Kinase, an Integral Membrane Protein. B. M. Gorzelle, J. K. Nagy, K. Oxenoid, W. L. Lonzer, D. S. Cafiso, and C. R. Sanders. Biochemistry 38, 16373-16382 (1999).

Distribution of phospholipids and triglycerides in multivesicular lipid particles. J. F. Ellena, M. Le, D. S. Cafiso, R. M. Solis, M. Langston, and M. B. Sankaram. Drug Delivery 6, 97-106 (1999).

Continuum Solvent Model Calculations of Alamethicin-Membrane Interactions: Thermodynamic Aspects. A. Kessel, D. S. Cafiso and N. Ben-Tal. Biophysical Journal 78, 571-583 (2000).

Substrate-induced exposure of an energy-coupling motif of a membrane transporter. H. Merianos, N. Cadieux, C. Lin, R. Kadner, D. Cafiso. Nature Struct. Biol. 7, 205-209 (2000).

Identifying conformational changes with site directed spin labeling. W. Hubbell, D. Cafiso, C. Altenbach. Nature Structural Biology 7, 735-739 (2000).

The secretory carrier membrane protein family: structure and membrane topology. C. Hubbard, D. Singleton, M. Rauch, S. Jayasinghe, D. Cafiso, J. Castle. Mol. Biol. Cell 11, 2933-2947 (2000).

Transport-Defective Mutations Alter the Conformation of the Energy Coupling Motif of an Outer Membrane Transporter. Coggshall, K. A., Cadieux, N., Piedmont, C., Kadner, R. J., Cafiso, D. S. Biochemistry 40, 13964-13971 (2001).

Location and Dynamics of Basic Peptides at the Membrane Interface: EPR spectroscopy of TOAC labeled peptides. K. Victor, D. Cafiso. Biophysical J. 81, 2241-2250 (2001).

Membrane Structure and Fusion-Triggering Conformational Change of the Fusion Domain from Influenza Hemagglutinin. H. Xing, J. H. Bushweller, D. S. Cafiso and Lukas K. Tamm. Nature Structure Biology. 8, 715-20 (2001).

Peptide-membrane interactions determined using site directed spin labeling. D. S. Cafiso. Current Topics in Membranes 52, 3-29 (2002).

Myristoylated Alanine-rich C Kinase Substrate (MARCKS) Sequesters Spin-labeled Phosphatidylinositol 4,5-Bisphosphate in Lipid Bilayers. Rauch ME, Ferguson CG, Prestwich GD, Cafiso DS. J Biol. Chem. 277, 14068-76 (2002).

Membrane Orientation and Position of the C2 Domain from cPLA2 by Site-Directed Spin Labeling. Frazier, A. A.; Wisner, M. A.; Malmberg, N. J.; Victor, K. G.; Fanucci, G. E.; Nalefski, E. A.; Falke, J. J.; Cafiso, D. S. Biochemistry 41, 6282-6292 (2002).

Perturbation of a very late step of regulated exocytosis by a secretory carrier membrane protein (SCAMP2)-derived peptide. Z. Guo, L. Liu, D. Cafiso, D. Castle. J. Biol. Chem. 277, 35357-35363 (2002).

Perfluorooctylbromide has limited membrane solubility and is located at the bilayer center. Locating small molecules in lipid bilayers through paramagnetic enhancements of NMR relaxation. J. F. Ellena, V. V. Obraztsov, V. L. Cumbea, C. M. Woods and D. S. Cafiso. J. Med. Chem. 45, 5534-5542 (2002).

Structure and Dynamics of the b-Barrel of the Membrane Transporter BtuB by Site-Directed Spin Labeling. G. E. Fanucci, N. Cadieux, C. A. Piedmont, R. J. Kadner, D. S. Cafiso. Biochemistry ; 41, 11543-11551 (2002).

Membrane bound orientation and position of the synaptotagmin I C2A domain by site-directed spin labeling. A. A. Frazier, C. R. Roller, J. J. Havelka, A. Hinderliter and D. S. Cafiso. Biochemistry, 42, 96-105 (2003).

Substrate-Induced Conformational Changes of the Perplasmic N-Terminus of an Outer-Membrane Transporter by Site-Directed Spin Labeling. G. E. Fanucci, K. A. Coggshall, N. Cadieux, M. Kim, R. J. Kadner and D. S. Cafiso. Biochemistry 42, 1391-1400 (2003).

Competing Ligands Stabilize Alternate Conformations of the Energy Coupling Motif of a TonB-Dependent Outer Membrane Transporter. G. E. Fanucci., N. Cadieux., R. J. Kadner., D. S. Cafiso. Proc. Natl. Acad. Sci. USA 100, 11382-11387 (2003).

Design, synthesis, and evaluation of analogues of 3,3,3-trifluoro-2-hydroxy-2-phenyl-propionamide as orally available general anesthetics. I. Choudhury-Mukherjee, H. A. Schenck, S. Cechova, T. N. Pajewski, J. Kapur, J. Ellena, D. S. Cafiso, and M. L. Brown. J Med Chem. 46, 2494-501 (2003).

Location of the myristoylated alanine-rich C-kinase substrate (MARCKS) effector domain in negatively charged phospholipid bicelles. J. F. Ellena, M. C. Burnitz and D. S. Cafiso. Biophys J. 2003, 85, 2442-8.

Spectroscopic Evidence that Osmolytes Used in Crystallization Buffers Inhibit a Conformation Change in a Membrane Protein. G. E. Fanucci, J. Y. Lee and D. S. Cafiso. Biochemistry 42, 13106-1312 (2003).

Differential substrate-induced signaling through the TonB-dependent transporter BtuB. N. Cadieux, P. G. Phan PG, D. S. Cafiso, and R. J. Kadner. Proc. Natl. Acad. Sci. U S A. 100, 10688-93 (2003).

Membrane Mimetic Environments Alter the Conformation of the Outer-Membrane protein BtuB. G. E. Fanucci, J. Y. Lee and D. S. Cafiso. J. Am. Chem. Soc. 125, 13932-933 (2003).

Electrostatic sequestration of PIP2 on phospholipid membranes by basic/aromatic regions of proteins. A. Gambhir, G. Hangyás-Mihályné I. Zaitseva, D. S. Cafiso, J. Wang, D. Murray, S. N. Pentyala, S. O. Smith, S. McLaughlin. In the press, Biophysical Journal. 86, 2188-2207 (2004).

Membrane Position of a Basic Aromatic Peptide that Sequesters Phosphatidylinositol 4,5 Bisphosphate Determined by Site-Directed Spin Labeling and High-Resolution NMR. J. F. Ellena, J. Moulthrop, J. Wu, M. Rauch, S. Jaysinghne, J. D. Castle, and D. S. Cafiso. Biophysical Journal in the press (2004).

Membrane Bound Orientation and Position of the Synaptotagmin C2B Domain Determined by Site-Directed Spin Labeling. E. Rufener, A. Frazier, C. M. Wieser, A. Hinderliter, and D. S. Cafiso. Biochemistry, in the press (2004).

Structure and Interactions of C2 domains at Membrane Surfaces.  D. S. Cafiso.  In: Protein-Lipid Interactions: From Membrane Domains to Cellular Networks Edited by Lukas K. Tamm WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. (2005).

Membrane structures of the hemifusion-inducing fusion peptide mutant G1S and the fusion-blocking mutant G1V of influenza virus hemagglutinin suggest a mechanism for pore opening in membrane fusion.  Y. Li, X. Han, A. L. Lai, J. H. Bushweller, D. S. Cafiso and Tamm, L. K. J. Virology 79, 12065-12076 (2005).

Solutes modify a conformational transition in a membrane transport protein.  Kim, M, Xu, Q, Fanucci, GE, Cafiso, DS.  Biophysical Journal, 90, 2922-2929 (2006).

 Position of Synaptotagmin I at the Membrane Interface:  Cooperative Interactions of Tandem C2 Domains.  Dawn Z. Herrick, S. Sterbling, K. A. Rasch, A. Hinderliter and D. S. Cafiso.  Biochemistry, 45, 9668-9674 (2006).

Recent Advances and Applications in Site-Directed Spin Labeling.  G. E. Fanucci and D. S. Cafiso.  Current Opinion in Structural Biology, 16, 1-10 (2006).

Substrate-Dependent Unfolding of the Energy Coupling Motif of a Membrane Transporter Protein Determined by Double Electron-Electron Resonance.  Q. Xu, J. F. Ellena, M. Kim and D. S. Cafiso.  Biochemistry, 45, 10847-10854 (2006).

Molecular Basis for Substrate-Dependent Transmembrane Signaling in an Outer-Membrane Transporter. Lukasik, S. M., Ho, K. W. D., and D. S. Cafiso.  J. Mol. Biol. 370, 807-811 (2007).

Substrate-dependent transmembrane signaling in TonB-dependent transporters is not conserved.  Kim, M., Fanucci, G. E., Cafiso, D. S.  Proc. Natl. Acad. Sci. 104, 11975-11980 (2007).

Secretory Carrier Membrane Protein SCAMP2 and Phosphatidylinositol-4,5-Bisphosphate Interactions in the Regulation of Dense Core Vesicle Exocytosis.  Liao, H., Ellena, J.,  Liu, L., Szabo, G., Cafiso, D., and D. Castle.  Biochemistry, 47, 670-679 (2007).

The structure of the coiled-coil domain of Ndel1 and the basis of its interaction with Lis1, the causal protein of Miller-Dieker lissencephaly.  Derewenda, U, Tarricone, C., Choi W. C., Cooper, D. R., Lukasik, S., Perrina, F., Tripathy, A., Kim, M. H., Cafiso, D. S., Musacchio, A., Derewenda, Z. S.  Structure 15, 1467-1468 (2007).

Intra and inter-molecular interactions dictate the aggregation state of irinotecan co-encapsulated with floxuridine inside liposomes.  Dicko A, Frazier AA, Liboiron BD, Hinderliter A, Ellena JF, Xie X, Cho C, Weber T, Tardi PG, Cabral-Lilly D, Cafiso DS, Mayer LD.  Pharm Res. 25, 1702-1713 (2008).

Solutes Alter the Conformation of the Ligand Binding Loops in Outer Membrane Transporters  Miyeon Kim, Qi Xu, David Murray, and David S. Cafiso.  Biochemistry 47, 670-679 (2008).

Membrane Hydrocarbon Thickness Modulates the Dynamics of a Membrane Transport Protein: Site-Directed Spin Labeling of a Membrane b-Barrel. Qi Xu, Miyeon Kim, K. W. David Ho, Gail E. Fanucci and David S. Cafiso.  Biophys. J., 95, 2849-2858 (2008)

NMR Solution Structure of the Integral Membrane Enzyme DsbB - Functional Insights into DsbB Catalyzed Disulfide Bond Formation.  Yunpeng Zhou, Tomasz Cierpicki, Stephen M. Lukasik, Ricardo H. Flores Jimenez, Jeffrey F. Ellena, David S. Cafiso, Jon Beckwith, and John H. Bushweller.  Mol Cell. 31, 896-908 (2008)

Spin-Diffusion Couples Proton Relaxation Rates for Proteins in Exchange with a Membrane Interface.  J. Magn. Reson., 194, 2849-2858 (2008).

Conformation and Membrane Position of the Region Linking the Two C2 Domains in Synaptotagmin 1 by Site-Directed Spin Labeling.  Hao Huang and David S. Cafiso, Biochemistry 47, 12380-12388 (2008).

Properties of Membrane Proteins.  Columbus, L. M., Nakamoto, R. K. and D. S. Cafiso.  Wiley Encyclopedia of Chemical Biology (2009)

The Calcium-Dependent and Calcium Independent Binding Modes of Synaptotagmin 1:  Two Modes of C2B Binding.  W. Kuo, D. Z. Herrick, J. F. Ellena, and D. S. Cafiso.  J. Mol. Biol. 387, 284-294 (2009).

Probing the conformation of the resting state of a bacterial multidrug ABC transporter, BmrA, by a site-directed spin labeling approach. M. A. Do Cao, S. Crouzy, M. Kim, M. Becchi, D. S. Cafiso, A. D. Pietro, and J. M. Jault.  Protein Sci. Apr 16 (2009)

Solution and membrane-bound conformations of the tandem C2A and C2B domains of synaptotagmin 1: Evidence for bilayer bridging.  Herrick DZ, Kuo W, Huang H, Schwieters CD, Ellena JF, Cafiso DS.   J Mol Biol. 2009 390: 913-23 (2009).

Dynamic structure of lipid-bound synaptobrevin suggests a nucleation-propagation mechanism for trans-SNARE complex formation.  Ellena JF, Liang B, Wiktor M, Stein A, Cafiso DS, Jahn R, Tamm LK.  Proc Natl Acad Sci U S A. 106(48):20306-11 (2009).

Osmolytes modulate conformational exchange in solvent-exposed regions of membrane proteins. Flores Jiménez RH, Do Cao MA, Kim M, Cafiso DS.  Protein Sci. 19:269-78 (2010).

Conformational exchange in a membrane transport protein is altered in protein crystals.  D. M. Freed, P. S. Horanyi, M. C. Wiener and D. S. Cafiso. Biophys J. 99:1604-10 (2010)

Synaptotagmin 1 and SNAREs Form a Complex That Is Structurally Heterogeneous. A. L. Lai, H. Huang, D. Z. Herrick, N. Epp and D. S. Cafiso.  J Mol Biol. 405: 696-706 (2011).

Membrane thickness varies around the circumference of the transmembrane protein BtuB.  Ellena JF, Lackowicz P, Mongomery H, Cafiso DS.  Biophysical Journal 100:1280-1287 (2011).

Phosphatidylinositol 4,5-bisphosphate alters synaptotagmin 1 membrane docking and drives opposing bilayers closer together.  Kuo W, Herrick DZ, Cafiso DS. Biochemistry. 50:2633-2641 (2011).

Partitioning of Synaptotagmin I C2 Domains between Liquid-Ordered and Liquid-Disordered Inner Leaflet Lipid Phases.  Wan C, Kiessling V, Cafiso DS, Tamm LK. Biochemistry. 50: 2478-2485 (2011). 

Solution structure of the ESCRT-I complex by small-angle X-ray scattering, EPR, and FRET spectroscopy.  Boura E, Rózycki B, Herrick DZ, Chung HS, Vecer J, Eaton WA, Cafiso DS, Hummer G, Hurley JH.  Proc Natl Acad Sci U S A. 108:9437-42 (2011).

Synaptotagmin 1 modulates lipid acyl chain order in lipid bilayers by demixing phosphatidylserine.  Lai AL, Tamm LK, Ellena JF, Cafiso DS.  J Biol Chem. 286:25291-300 (2011).

Molecular origin of electron paramagnetic resonance line shapes on β-barrel membrane proteins: the local solvation environment modulates spin-label configuration.  Freed DM, Khan AK, Horanyi PS, Cafiso DS.  Biochemistry. 50:8792-803 (2011).

Lipid and membrane mimetic environments modulate spin label side chain configuration in the outer membrane protein A.  Jiménez RH, Freed DM, Cafiso DS.  J Phys Chem B. 115:14822-30 (2011).