Stock Solutions, Ordering Info.

• dNTPs
  • dNTPs come in separate tubes from Perkin Elmer (Part # N808-0007) at a concentration of 10mM.
  • We make a 2.5 mM (each) stock by mixing those four tubes in equal volumes.
  • Combine 300 ul from each of the four tubes, vortex well.
  • Dispense into 50ul aliquots, and store in the general reagent box in the fridge.
• Long Ranger Sequencing Gels
  • Re-order from BMA, Type 377-36cm WTR, Cat # 50691.
  • Order 10 boxes at a time.
• Sequencing Kits
  • Re-order Applied Biosystems Part # 4303152
• Taq
  • Re-order Perkin Elmer Taq in 6 packs (Part # N808-0167)
• TBE
  • Starting with ~1 liter of water in a large beaker with a stir bar, add...
    • 86.4 g Tris
    • 43.2 g Boris Acid
    • 64 ml 0.5M EDTA (8.0)
  • After the Boric is finally dissoved, add the solution to an empty carboy and top it up to the 8 liter mark. Mix.

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