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6-His Protein Prep

Mike's Xenopus CSF Extracts

Mike's Xenopus Cycling Extracts

Sara's Xenopus CSF Extracts

Sara's Xenopus Cycling Extract

Assaying H1 kinase in extracts

Kinetochore-Microtubule Binding Assay in vitro

Immunoprecipitation

Isolating Mitotic Chromosomes

Isolating Mitotic Chromosomes - Desai Method

Silver Staining Protein Gels

Xenopus Tissue Culture Cell Lysates

Purifying Recombinant  Chromosomal Passenger Complex

Purifying GST-tagged Proteins

Immunofluorescence with Biotinylated Antibodies

Test Inductions of Recombinant Proteins

Induction of Recombinant Proteins

In Vitro Kinase Assays

Xenopus Tissue Culture

 

 

This page last modified 08/11/2011

The Stukenberg Lab and the Burke Lab are in the Department of Biochemistry and Molecular Genetics at the University of Virginia