Highlights of classic papers "from the Vault" |
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Cytoskeletal Milestones: First (focal) contact (Nature 2008) |
Pursuing the middleman: the hunt for integrins (JCB 2005) |
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Click image to download .pdf from Nature website |
Click image to download .pdf from JCB website |
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Recent Publications |
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Hanein D, Horwitz AR (2011) The structure of cell-matrix adhesions: the new frontier. Curr Opin Cell Biol. [Epub ahead of print] |
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Vicente-Manzanares M, Horwitz AR (2011) Adhesion dynamics at a glance. J Cell Sci. 124(Pt 23):3923-7. No abstract available. |
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Hodges JL, Newell-Litwa K, Asmussen H, Vicente-Manzanares M, Horwitz AR (2011) Myosin IIb activity and phosphorylation status determines dendritic spine and post-synaptic density morphology. PLoS One. 6(8):e24149. Abstract (Pubmed) |
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Huttenlocher A, Horwitz AR (2011) Integrins in cell migration. Cold Spring Harb Perspect Biol. 3(9). pii: a005074. Abstract (Pubmed) |
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Lamers ML, Almeida ME, Vicente-Manzanares M, Horwitz AF, Santos MF (2011) High glucose-mediated oxidative stress impairs cell migration. PLoS One. 6(8): e22865. Abstract (Pubmed) |
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Newell-Litwa KA, Horwitz AR (2011) Cell Migration: PKA and RhoA Set the Pace. Curr Biol. 21(15): R596-8. Abstract (Pubmed) |
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Vicente-Manzanares M, Horwitz AR (2011) Cell Migration: an overview. Methods Mol Biol. 769: 1-24. Abstract (Pubmed) |
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Vicente-Manzanares M, Newell-Litwa K, Bachir AI, Whitmore LA, Horwitz AR (2011) Myosin IIA/IIB restrict adhesive and protrusive signaling to generate front-back polarity in migrating cells. J Cell Biol. 193(2): 381-96. Abstract (Pubmed) |
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Choi CK, Zareno J, Digman MA, Gratton E, Horwitz AR (2011) Cross-Correlated Fluctuation Analysis Reveals Phosphorylation-Regulated Paxillin-FAK Complexes in Nascent Adhesions. Biophys J. 100(3): 583-92. Abstract (Pubmed) |
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Kubow K, Horwitz AR (2011) Reducing background fluorescence reveals adhesions in 3D matrices. Nat Cell Bio. 13(1): 3-5. No abstract available. |
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Vicente-Manzanares M, Horwitz AR (2010) Myosin light chain mono- and di-phosphorylation differentially regulate adhesion and polarity in migrating cells. Biochem Biophys Res Commun. 402(3): 537-42. Abstract (Pubmed) |
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Parsons JT, Horwitz AR, Schwartz MA (2010) Cell adhesion: integrating cytoskeletal dynamics and cellular tension. Nat Rev Mol Cell Biol. 11(9): 633-43. Review. Abstract (Pubmed) |
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Cirit M, Krajcovic M, Choi, CK, Erik S. Welf ES, Horwitz AF, Haugh JM (2010) Stochastic Model of Integrin-Mediated Signaling and Adhesion Dynamics at the Leading Edges of Migrating Cells. PLOS Computational Biology. 6(2): e1000688. Abstract (Pubmed) |
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Vicente-Manzanares M, Hodges J, Horwitz AR (2009) Dendritic spines: Similarities to adhesions and protrusions in migrating cells. The Open Neuroscience Journal. 3: 87-96. |
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Vicente-Manzanares M, Ma X, Adelstein RS, Horwitz AR (2009) Non-muscle myosin II takes centre stage in cell adhesion and migration. Nat Rev Mol Cell Biol. 10(11): 778-90. Review. Abstract (Pubmed) |
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Hall EH, Daugherty AE, Choi CK, Horwitz AF, Brautigan DL (2009)Tensin1 requires protein phosphatase 1a in addition to RHO(GAP) DLC-1 to control cell polarization, migration, and invasion. J Biol Chem. 284(50): 34713-22. Abstract (Pubmed) |
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Horwitz AR (2009) The matrix revolutions. Nat Rev Mol Cell Biol. 10(10): 653. No abstract available. |
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Digman MA, Wiseman PW, Choi C, Horwitz AR, Gratton E (2009) Stoichiometry of molecular complexes at adhesions in living cells. PNAS. 106(7): 2170-5. Abstract (Pubmed) |
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Vicente-Manzanares M, Choi CK, Horwitz AR (2009) Integrins in cell migration: the actin connection. Journal of Cell Science. 122(Pt 2): 199-206. Abstract (Pubmed) |
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Digman MA, Wiseman PW, Horwitz AR, Gratton E (2009) Detecting protein complexes in living cells from laser scanning confocal image sequences by the ccRICS method. Biophysical Journal. 96(2): 707-16. Abstract (Pubmed) |
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Vicente-Manzanares M, Koach MA, Whitmore L, Lamers ML, Horwitz AF (2008) Segregation and activation of myosin IIB creates a rear in migrating cells. Journal of Cell Biology. 183(3): 543-54. Abstract (Pubmed) |
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Macdonald A, Horwitz AR, Lauffenburger DA (2008) Kinetic model for lamellipodial actin-integrin 'clutch' dynamics. Cell Adhesion & Migration. 2(2): 95-105. Abstract (Pubmed) |
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Choi CK, Vicente-Manzanares M, Zareno J, Whitmore L, Mogilner A, Horwitz AF (2008) Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor independent manner. Nature Cell Bio. 10: 1039-1050. Abstract (Pubmed) |
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Pfister A, Zhang G, Zareno J, Horwitz AF, Fraser C (2008) Boron polylactide nanoparticles exhibiting fluorescence and phosphorescence in aqueous medium. ACS Nano. 2(6): 1252-1258. Abstract (Pubmed |
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Easley IV CA, Brown CM, Horwitz AF, Tombes RM (2008) CaMK-II promotes focal adhesion turnover and cell motility by inducing tyrosine dephosphorylation of FAK and Paxillin. Cell Motility and Cytoskeleton. 65(8):662-674. Abstract (Pubmed) |
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Murase S, Cho C, White JM, Horwitz AF (2008) ADAM2 promotes migration of neuroblasts in the rostral migratory stream to the olfactory bulb. Eur J of Neuro. 27(7): 1585-95. Abstract (Pubmed) |
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Brown CM, Dalal RB, Hebert B, Digman MA, Horwitz AR, Gratton E (2008) Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope. J Microsc. 229: 78-91. Abstract (Pubmed) |
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Digman MA, Dalal R, Horwitz AF, Gratton E (2007) Mapping the number of molecules and brightness in the laser scanning microscope. Biophys J. 94(6):2320-32. Abstract (Pubmed) |
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Owen KA, Pixley FJ, Thomas KS, Vicente-Manzanares M, Ray BJ, Horwitz AF, Parsons JT, Beggs HE, Stanley ER, Bouton AH (2007) Regulation of lamellipodial persistence, adhesion turnover, and motility in macrophages by focal adhesion kinase. J Cell Biology. 179(6): 1275-87. Abstract (Pubmed) |
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Digman MA, Brown CM, Horwitz AF, Mantulin WW, Gratton E (2007) Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy. Biophys J. 94(7):2819-31. Abstract (Pubmed) |
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Mayhew MW, Jeffery ED, Sherman NE, Nelson K, Polefrone JM, Pratt SJ, Shabanowitz J, Parsons JT, Fox JW, Hunt DF, Horwitz AF (2007) Identification of phosphorylation sites in BetaPIX and PAK1. J. Cell Science. 120: 3911-3918. Abstract (Pubmed) |
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Dalal RB, Digman MA, Horwitz AF, Vetri V, Gratton E (2007) Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode. Microsc Res Tech. 71(1):69-81.Abstract (Pubmed) |
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Vicente-Manzanares M, Zareno J, Whitmore L, Choi CK, Horwitz AF (2007) Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells.176(5): 573-80. Abstract (Pubmed) |
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Huttenlocher A and Horwitz AR (2007) Wound healing with electric potential. New England J or Medicine. 356(3): 303-4. Abstract (Pubmed) |
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Webb DJ, Zhang H, Majumdar D, Horwitz AF (2007) Alpha 5 integrin signaling regulates the formation of spines and synapses in hippocampul neurons. J Biol Chem. 282(10: 6929-35. Abstract (Pubmed) |
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Brown CM, Hebert B, Kolin DL, Zareno J, Whitmore L, Horwitz AR, Wiseman PW (2006) Probing the integrin-actin linkage using high-resolution protein velocity mapping. J. Cell Science. 119(Pt 24): 5204-14. Abstract (Pubmed) |
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Mayhew MW, Webb DJ, Kovalenko M, Whitmore L, Fox JW, Horwitz AF (2006) Identification of Protein Networks Associated with the PAK1-BetaPIX-GIT1-Paxillin Signaling Complex by Mass Spectrometry. J of Proteome Research.5(9): 2417-2423. Abstract (Pubmed) |
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Sakakibara A and Horwitz AF (2006) Mechanism of polarized protrusion formation on neuronal precursors migrating in the developing chicken cerebellum. J. Cell Science. 119(Pt 17): 3583-92. Abstract (Pubmed) |
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Wang YL, Hahn KM, Murphy RF, Horwitz AF (2006) From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19-21, 2006. J. Cell Biol. 174(4): 481-4. Abstract (Pubmed) |
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Webb DJ, Mayhew MW, Kovalenko M, Schroeder MJ, Jeffery ED, Whitmore L, Shabanowitz J, Hunt DF, Horwitz AF (2006) Identification of phosphorylation sites in GIT1. J Cell Science. 119(Pt 14): 2847-50. Abstract (Pubmed) |
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Schwartz MA and Horwitz AR (2006) Integrating adhesion, protrusion, and contraction during cell migration. Cell. 125(7): 1223-1225. Abstract (Pubmed) |
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Webb DJ, Kovalenko M, Whitmore L, Horwitz AF (2006) Phosphorylation of serine 709 in GIT1 regulates protrusive activity in cells. Biochem Biophys Res Commun. 346(4): 1284-8. Abstract (Pubmed) |
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Nayal A, Webb DJ, Brown CM, Schaefer EM, Vicente-Manzanares M, Horwitz AR (2006) Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics. J Cell Biol. 173(4): 587-9. Abstract (Pubmed) |
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Moissoglu K, Slepchenko BM, Meller N, Horwitz AF, Scwartz MA (2006) In Vivo Dynamics of Rac-Membrane Interactions. Mol Biol Cell. 17(6): 2770-9. Abstract (Pubmed) |
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Vicente-Manzanares M, Webb DJ, Horwitz AR (2005) Cell migration at a glance. J Cell Sci. 118(Pt 1): 4917-9. Abstract (Pubmed) Download Hi-Res Poster here. |
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Webb DJ, Schroeder MJ, Brame CJ, Whitmore L, Shabanowitz J, Hunt DF, Horwitz AR (2005) Paxillin phosphorylation sites mapped by mass spectroscopy. J Cell Sci. 118(Pt 1): 4925-9. Abstract (Pubmed) |
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Schroeder MJ, Webb DJ, Shabanowitz J, Horwitz AF, Hunt DF (2005) Methods for the detection of paxillin post-translational modifications and interacting proteins by mass spectroscopy. J Proteome Res. 4(5): 1832-4. Abstract (Pubmed) |
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Digman MA, Brown CM, Sengupta P, Wiseman PW, Horwitz AR, Gratton E (2005) Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophys J. 89(2): 1317-1327. Abstract (Pubmed) |
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Carbonell WS, Murase S, Horwitz AF, Mandell JW (2005) Migration of perilesional microglia after focal brain injury and modulation by CC chemokine receptor 5: an in situ time-lapse confocal imaging study. J of Neuroscience. 25(30): 7040-7047. Abstract (Pubmed) |
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Zhang H, Webb DJ, Asmussen H, Niu S, Horwitz AF (2005) A GIT1/PIX/Rac/PAK/ Signaling module regulates spine morphogenesis and synapse formation through MLC. J of Neuroscience. 25(13): 3379-3388. Abstract (Pubmed) |
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Digman MA, Sengupta P, Wiseman PW, Brown CM, Horwitz AR, Gratton E (2005) Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure. Biophys J. 88(5): L33-6. Abstract (Pubmed) |
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Harms BD, Bassi GM, Horwitz AR, Lauffenburger DA (2005) Directional persistence of EGF-induced cell migration is associated with stabilization of lamellipodial protrusions. Biophys J. 88(2): 1479-88. Abstract (PubMed) |
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Carbonell WS, Murase S, Horwitz AF, Mandell JW (2005) Infiltrative microgliosis: activation and long-distance migration of subependymal microglia following periventricular insults. J of Neuroinflamation. 2(1): 5. Abstract (PubMed) |
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Wiseman PW, Brown CM, Webb DJ, Herbert B, Johnson NL, Squier JA, Ellisman MH, Horwitz AF (2004) Spatial mapping of integrin interactions and dynamics during cell migration by image correlation microcopy. J Cell Science. 117(Pt 23): 5521-5534. Abstract (PubMed) |
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Webb DJ, Donais K, Whitmore LA, Thomas SM, Turner CE, Parsons JT, Horwitz AF (2004) FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion diassembly. Nat Cell Biol. 6(2): 154-161. Abstract (PubMed) |
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Nayal A, Webb DJ, and Horwitz AF. (2004) Talin: an emering focal point of adhesion dynamics. Curr Opin Cell Biol. 16(1) :94-8. Abstract (PubMed) |
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Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR (2003) Cell migration: integrating signals from front to back. Science. 1704-1709. Review. Abstract (PubMed) |
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Horwitz AR. (2003) Building bridges through collaboration--a pathway for interdisciplinary research. Trends Cell Biol.13(1):2-3. No abstract available. |
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Webb DJ, Brown CM, Horwitz AF. (2003) Illuminating adhesion complexes in migrating cells: moving toward a bright future. Curr Opin Cell Biol. 15(5):614-20. Abstract (Pubmed) |
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Webb DJ, Horwitz AF. (2003) New dimensions in cell migration. Nat Cell Biol. 5(8):690-2. No abstract available. |
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Zhang H, Webb DJ, Asmussen H, and Horwitz AF. (2003) Synapse formation in regulated by the signaling adaptor GIT1. J.Cell Biol; 161(1):131-42. Abstract (Pubmed) |
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Horwitz R, Webb D.(2003) Cell migration. Curr Biol.13(19):R756-9. Review. No abstract available |
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Manabe R, Whitmore L, Weiss JM, Horwitz AR. (2002) Identification of a novel microtubule-associated protein that regulates microtubule organization and cytokinesis by using a GFP-screening strategy. Curr Biol.12(22):1946-51. Abstract (Pubmed) |
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Mandell JW, Manabe R, Horwitz AF, Baumgart JP. (2002) Fluorescence imaging of mobility shifts: an expression cloning method for identification of cell signaling targets. Lab Invest. 82(12):1631-6. Abstract (Pubmed) |
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Webb DJ, Asmussen H, Murase S, and Horwitz AF (2002) Cell migration in slice culture. Methods Cell Biol. 69:341-58. No abstract available. |
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Murase S and Horwitz AF (2002) Deleted in colorectal carcinoma and differentially expressed integrins mediate the directional migration of neural precursors in the rostral migratory stream. J Neurosci. May 1; 22 (9) :3568-79. Abstract (Pubmed) |
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Webb DJ, Parsons JT, and Horwitz AF (2002) Adhesion assembly, disassembly and turnover in migrating cells--over and over and over again. Nat Cell Biol. 4(4) :E97-100. Review. Abstract (Pubmed) |
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Manabe R, Kovalenko M, Webb DJ, and Horwitz AR (2002) GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J Cell Sci. 115 (Pt 7) :1497-510. Abstract (Pubmed) |
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Laukaitis CM, Webb DJ, Donais K, and Horwitz AF (2001) Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. J Cell Biol. Jun 25; 153 (7) :1427-40. Abstract (Pubmed) |
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Knight B, Laukaitis C, Akhtar N, Hotchin NA, and Horwitz AF (2000) Visualizing cell migration in situ. Current Biology. 10: 576-585. Abstract (Pubmed) |
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Asthagiri AR, Reinhart CA, Horwitz AF, and Lauffenburger DA (2000) Transient ERK2 activation quantitatively governs fibronectin and insulin-mediated co-regulation of DNA synthesis. J Cell Science. 113(Pt24):4499-4510. Abstract (Pubmed) |
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Asthagiri AR, Nelson CM, Horwitz AF, and Lauffenburger DA (1999) Quantitative relationship among integrin-ligand binding, adhesion, and signalling via FAK and ERK2. J Biol Chem. 274:27119-27. Abstract (Pubmed) |
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Ashthagiri AR, Horwitz AF, and Lauffenburger DA (1999) A rapid and sensitive quantitative kinase activity assay using a convenient 96-well format. Anal Biochem. 269(2): 342-7. Abstract (Pubmed). |
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Horwitz AR and Parsons JT, (1999) Cell Migration- Movin' on. Science. 286: 1102-1103. No abstract available. |
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Sastry S, Lakonishok M, Wu Stanley S, Trong T, Huttenlocher A, Turner C, and Horwitz AF (1999) Quantitative changes in integrin and focal adhesion signalling regulate myoblast cell cycle withdrawal. J Cell Biol. 144: 1295-1309. Abstract (Pubmed) |
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Cao Z, Huang K, and Horwitz AF (1998) Identification of a domain on the integrin alpha5 subunit implicated in cell spreading and signaling. J Biol Chem. 273: 31670-31679. Abstract (Pubmed) |
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Huttenlocher A, Kinder M, Lakonishok M, Knudsen K, and Horwitz A (1998) Integrin and cadherin synergy regulates contact inhibition of migration and motile activity. J Cell Biol. 141: 515-526. Abstract (Pubmed) |
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Palecek SP, Huttenlocher A, Horwitz AF, and Lauffenburger DA (1998) Physical and biochemical regulation of intern release during rear detachment of migrating cells. J Cell Sci. 111: 929-940. Abstract (Pubmed) |
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Horwitz AF (1997) Integrins and Health. Scientific American. 276: 68-75. No abstract available. |
"from the Vault" |
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Huttenlocher, A., Palecek, S.P., Lauffenburger, D.A., Ginsberg, M.H., Lin, Q., Zhang, W., Mellgren, R., and Horwitz, A.F. (1997) Regulation of cell migration by the Ca++ dependent protease, calpain. J Biol Chem. 272: 32719-32722. |
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Palecek, S., Loftus, J.C., Ginsberg, M.H., Lauffenburger, D.L., and Horwitz, A.F. (1997) Integrin -ligand binding properties govern cell migration speed through cell-substratum adhesiveness. Nature. 385: 537-540. |
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Sastry, S., Lakonishok, M., and Horwitz, A.F. (1996) Integrin alpha subunit ratios, cytoplasmic domains, and growth factor synergy regulate muscle proliferation and differentiation: J Cell Biol. 133: 169-184. |
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Lauffenburger, D. and Horwitz, A. (1996) Cell Migration: A physically integrated molecular process. Cell 84: 1-20. |
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Huttenlocher A, Sandborg RR, Horwitz AF (1995) Adhesion in cell migration. Curr Opin Cell Biol. 7(5): 697-706. |
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Lilienbaum A, Reszka AA, Horwitz AF, Holt CE (1995) Chimeric integrins expressed in retinal ganglion cells impair process outgrowth in vivo. Mol Cell Neurosci. 6(2): 139-52. |
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Bao, Z.Z., Lakonishok, M., Kaufman, S., and Horwitz, A.F. (1993) The alpha7 beta1 integrin is a component of the myotendinous junction in skeletal muscle. J Cell Sci. 106: 579-590. |
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Schmidt, C.F., Horwitz, A.F., Lauffenburger, D.A., and Sheetz, M.P. (1993) Integrin cytoskeletal interactions are dynamic, asymmetric and regulated. J Cell Biol. 123: 977-991. |
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Sastry, S.K. and Horwitz, A.F. (1993) Integrin cytoplasmic domains: Mediators of cytoskeletal linkages of extra- and intracellular signaling. Curr Opin Cell Biol. 5: 819-831. |
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Galileo DS, Majors J, Horwitz AF, Sanes JR (1992) Retrovirally introduced antisense integrin RNA inhibits neuroblast migration in vivo. Neuron. 9(6): 1117-31. |
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Regen, C.M. and Horwitz, A. (1992) Dynamics of beta1 integrin-mediated adhesive contacts in motile fibroblasts. J Cell Biol. 119: 1347-1359. |
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Reszka, A., Hayashi, Y. and Horwitz, A., (1992) Identification of amino acid sequences in the integrin beta 1 cytoplasmic domain implicated in cytoskeletal association. J Cell Biol. 117: 1321-1330. |
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Muschler JL and Horwitz AF (1991) Down-regulation of the chicken alpha 5 beta 1 integrin fibronectin receptor during development. Development. 113(1): 327-37. |
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Tapley P, Horwitz A, Buck C, Duggan K, Rohrschneider L (1989) Integrins isolated from Rous sarcoma virus-transformed chicken embryo fibroblasts. Oncogene. 4(3): 325-33. |
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Jaffredo T, Horwitz AF, Buck CA, Rong PM, Dieterlen-Lievre F (1988) Myboblast migration specifically inhibited in the chick embryo by grafted CSAT hybridoma cells secreting an anti-integrin antibody. Development. 46(2): 431-36. |
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Tamkun, J. W., DeSimone, D. W., Fonda, D., Patel, R. S., Buck, C., Horwitz, A. and Hynes, R. O. (1986) Structure of integrin: a glycoprotein involved in transmembrane linkage between fibronectin and actin. Cell. 46: 271-282. |
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Horwitz, A., Duggan, K., Buck, C., Beckerle, M., and Burridge, K. (1986) Interaction of plasma membrane fibronectin receptor with talin-a transmembrane linkage. Nature 320: 531-533. |
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Bozyczko, D. and Horwitz, A. (1986) The participation of a putative fibronectin and laminin receptor in neurite extension. J Neurosci. 6: 1241-1251. |
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Neff, N. T., Lowrey, C., Decker, C., Tovar, A., Damsky, C., Buck, C., and Horwitz, A. (1982) A monoclonal antibody detaches embryonic skeletal muscle from extracelluar matrices. J Cell Biol. 95: 654-666. |
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Knudsen, K., and Horwitz, A. F. (1977) Tandem events in myoblast fusion. Dev Biol. 58: 328-338. |
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Horwitz, A. F., Hatten, M. E., and Burger, M. M. (1974) Membrane fatty acid replacement and their effect on growth and lectin-induced agglutinability: suggestion of a heterogeneous lipid phase. Proc Natl Acad Sci. 71: 3115-3119. |
